A 1 M sodium acetate buffer adjusted to pH 5.0 at room temperature and tested for protease activity is intended for biochemical workflows that require acetate as a counterion and stable acidic pH. The formulation is used in nucleic acid precipitation and purification, ion-exchange chromatography, acidic enzyme assays and selected protein applications. Its use is constrained by the effective buffering range of acetate systems, which generally spans pH 3.6 to 5.6.
The buffer is formulated at 1 M concentration and adjusted to pH 5.0 at room temperature. It is designed to provide pH stability in processes where acetate serves as the counterion, according to the product information.
The solution is subjected to protease activity testing. That step is intended to protect samples sensitive to protein degradation and to support the reliability of experimental data, particularly when trace contamination could affect low-abundance proteins or degradation-sensitive materials.
The buffer is used where acidic pH and acetate counterions are required. Its principal applications and technical roles are summarized below.
| Application | Technical role |
|---|---|
| Nucleic acid precipitation | In acidic conditions, used with ethanol or isopropanol for efficient recovery of DNA or RNA. |
| Nucleic acid purification | Removes contaminants in salt- or acid-based purification protocols and supports nucleic acid concentration. |
| Ion-exchange chromatography | Serves as a low-pH buffer to maintain protein charge states and optimize elution and separation. |
| Enzyme activity assays | Provides a suitable kinetic environment for acidic enzymes such as glycosidases and acid phosphatases. |
| Protein formulation | Protects acid-stable proteins and peptides and supports long-term storage stability. |
| EDC coupling | Supports carbodiimide coupling reactions under the weakly acidic conditions required for carboxyl activation. |
When workflows involve low-abundance proteins or samples highly sensitive to degradation, the purity of the buffer itself becomes a critical variable. Untested reagents may introduce trace protease contamination and cause irreversible deviations in experimental results.
The 1 M sodium acetate buffer is verified for protease activity. The testing is intended to reduce the risk of introducing protease contamination during sample preparation, dialysis and buffer exchange.
Although the buffer is supplied at pH 5.0, several handling points require attention in laboratory use.
담당자: Mr. Jenkins Hu / Jinxu Hu
전화 번호: 15263616103
팩스: 86--0536-3524268
Korean
